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Fluoresence Reference Set

(6BF) Fluorescence Reference Set, PMMA blocks

Molecular fluorescence spectroscopy is a sen­sitive and often selective technique. Unlike absorption spectrophotometry, it is not an absolute technique: instruments therefore require calibration before every series of measurements. This may be achieved using a stable reference material, which should absorb and emit at similar wavelengths to the samples of interest. Use of the general purpose fluorescent reference material set type 6BF enables the day to day stability of fluorescence instruments to be verified.

The 6BF reference materials are not standards with absolute values, but a set of six relatively stable fluorescent materials in a polymethyl-methacrylate matrix with which the stability of the instrument can be monitored. Four blocks exhibit broad band spectra which cover the normally used UV and visible region of the spectrum. Two blocks contain materials suitable for illustrating the selectivity of the technique as well as checking instrument resolution and wavelength calibration.

Brief Overview of reference block contents:

Sample# Compound Nominal Concentration Excitation(nm) Emission(nm)
1 Anthracene 1x10-5 360 402
1 Napthalene 6x10-5 290 330
2 Ovalene 2x10-7 342 482
3 p-Terphenyl 5x10-7 295 338
4 Tetraphenylbutadiene 3x10-7 348 422
5 Compound 610 1x10-6 440 475
6 Rhodamine 2x10-7 562 573

How to Order

Catalog Number Description  
6BF Fluorescence Referene Set, 6 PMMA blocks  
#1 of 6BF Anthracine and Napthalene block  
#2 of 6BF Ovalene block  
#3 of 6BF p-Terphenyl block  
#4 of 6BF Tetraphenylbutadiene block  
#5 of 6BF Compound 610 block  
#6 of 6BF Rhodamine block