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The UV/Vis Holmium Oxide Wavelength Reference contains Holmium oxide (4%) in perchloric acid (10%) NIST Traceable with UKAS ISO 17025 accredited certificate of calibration. Its primary use is for the assessment of wavelength scale accuracy in both UV and Visible, with a usable range of 240nm to 650nm. This references contains a filled far UV quartz cell that has been permanently heat sealed
Holmium has been used as a wavelength standard for many years and now we have extended the use by creating a filled and sealed far UV quartz cell which allows it to be used into the UV spectra. Holmium offers sharp, stable peaks over the range of 219 to 650nm. Holmium perchlorate is used to assure that the wavelength scale of your instrument is within the manufacturer's tolerances to the actual wavelength being measured. The use of the filled quartz cell assures that the optical configuration for your quality control is exactly the same as for a normal analysis.
Holmium presents a wide range of crisply resolvable peaks which are easily used to correlate the wavelength indicator on your spectrophotometer to the known peak. The first procedure should be to scan the cell over the usable range to assure that all peaks can be resolved. Adjust the slit width and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances of the wavelength readout. If not, get a service technician to adjust or recheck your instrument. Holmium is measured against an air blank. Periodically check the spectra, at a minimum in the area where you are doing your analysis. Periodic use of the Holmium cell will build a log of your instrument’s spectral accuracy for use with certification and troubleshooting should the correlation change over time.